Retraction Notice to: Cytohesins Are Cytoplasmic ErbB Receptor Activators
نویسندگان
چکیده
(Cell 143, 201–211; October 15, 2010) In Bill et al., we identified the cytohesin ARNO as an ErbB receptor activator that enhances receptor autophosphorylation in H460 and SkBr3 cells. Cell-free reconstitution with purified proteins showed that ARNO enhances EGF receptor autophosphorylation by direct interaction with the intracellular domain of the receptor (EGFR-ICD). Wewere recently notified byCell that the laboratory of Dr. Oreste Segatto was unable to replicate these effects in HeLa cells or under cell-free reconstituted conditions. Although we reproducibly observe 1.6-fold ARNO-dependent stimulation of cellular EGFR autophosphorylation and partial colocalization of ARNO with EGFR in plasma membrane sheets of HeLa cells, we cannot reproduce the data showing direct stimulation of EGFR-ICD autophosphorylation by ARNO-Sec7 in a cell-free reconstitution system (shown in Figures 5D and S5D). Thus, the conclusion that ARNO enhances receptor activation by direct interaction with EGFR-ICD is no longer supported by experimental evidence. We maintain the view that cellular EGFR autophosphorylation is enhanced by ARNO, that ARNO and EGFR colocalize at the plasma membrane, and that ARNO binds to the EGFR-ICD. Nevertheless, our inability to reproduce the data in Figures 5D and S5D compromises our confidence in the proposed mechanism of this regulation. Therefore, we wish to retract the paper. We apologize for any inconvenience that may have resulted from its publication. Franziska Thorwith could not be reached about this retraction.
منابع مشابه
RETRACTED: Cytohesins Are Cytoplasmic ErbB Receptor Activators
Signaling by ErbB receptors requires the activation of their cytoplasmic kinase domains, which is initiated by ligand binding to the receptor ectodomains. Cytoplasmic factors contributing to the activation are unknown. Here we identify members of the cytohesin protein family as such factors. Cytohesin inhibition decreased ErbB receptor autophosphorylation and signaling, whereas cytohesin overex...
متن کاملFunction and mode of action of cytohesins in the epidermal growth factor pathway in colorectal cancer cells
Cytohesins have been identified as cytoplasmic ErbB receptor activators in certain cancers, exhibiting an important role in ErbB signaling. However, whether cytohesins are essential in colorectal cancer is unknown. The aim of the present study was to investigate whether cytohesins contribute to the epidermal growth factor (EGF) pathway in colorectal cancer cells. RT-PCR and immunofluorescence e...
متن کاملAnti-Proliferative Effect of Cytohesin Inhibition in Gefitinib-Resistant Lung Cancer Cells
Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKI), such as gefitinib, have been proven to efficiently inhibit the proliferation of a subset of non small-cell lung cancers (NSCLC). Unfortunately, the majority of NSCLC expressing wild type EGFR is primarily resistant to EGFR-TKI treatment. Here, we show that the proliferation of the gefitinib-resistant NSCLC cell lines H460...
متن کاملCytohesins/ARNO: The Function in Colorectal Cancer Cells
Epidermal growth factor (EGF) and insulin-like growth factor-I (IGF-I) are critical regulators of cell differentiation, survival, proliferation, and migration in cancers. This study found that ARNO (cytohesin-2), an activator of the EGF and IGF-I pathways, was more highly expressed in colorectal cancer tissue than in benign adjacent colorectal tissue. When ARNO-siRNA or the chemical inhibitor S...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Cell
دوره 165 شماره
صفحات -
تاریخ انتشار 2016